FastQCFastQC Report
Sun 21 May 2023
EGAF00007940957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400645
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA69511.7349523892722984No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57801.4426736886770084No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA35260.8800808695977735No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA30780.7682611788491058No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG24540.6125123238777471No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT24300.6065219833019256No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC23840.5950404971982678No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23190.5788166581387513No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG22360.5581000636473686No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG22190.5538569057394951No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22070.5508617354515843No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC21370.533389908772105No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC21270.5308939335321793No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC16930.42256860811940744No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16870.42107102297545207No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13160.3284703415742116No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12390.3092513322267843No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11530.287785945163424No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10170.25384068190043557No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9980.24909832894457687No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9760.2436071834167405No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC9700.2421095982727851No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9300.23212569731308266No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8920.22264099140136528No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT8840.2206442112094248No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8320.20766513996181157No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7920.1976812390021091No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7610.18994371575833968No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG7260.18120780241860002No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA7170.17896142470266693No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6720.16772953612300168No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC6190.15450086735139587No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6000.1497585143955372No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5970.1490097218235595No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5860.14626414905964133No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5740.1432689787717306No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5590.13952501591184216No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5480.13677944314792398No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5320.132785882764043No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC5300.13228668771605787No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5240.13078910257210247No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA5050.12604674961624382No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5030.1255475545682587No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4910.12255238428034794No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC4730.11805962884848181No Hit
AACACCCAGGAACGCATGGAGCTGCGTAAAC4630.11556365360855621No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG4620.11531405608456365No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC4600.11481486103657851No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4320.10782613036478678No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA4090.10208538731295785No Hit
TCCCACGAGGAACAGCCACGTTATGAAGACC4080.10183578978896529No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA4010.10008860712101737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)