FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007940961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405739
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA50571.2463677388666112No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37620.9271970404619719No Hit
ATGTCCAACATGGACATCGATGGTATTAACA36510.8998395520272885No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG32460.8000216888196598No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT29380.7241108200099078No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24910.6139414746918586No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24490.6035899925814379No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24370.6006324262641747No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23060.5683456606340529No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21030.5183134971003527No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG20120.4958852858611078No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19900.4904630809461255No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG19460.47961867111616086No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA18970.46754194198733673No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15700.3869482598419181No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13980.3445564759611474No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13250.3265646141977971No Hit
CCGGTAGCTCCGCAGCAGCAGTACCAGCAGC12680.3125161741907975No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12580.3100515355930783No Hit
CCGTGTAAAGACGCGAACGAATGCCATCTGA11700.2883627159331491No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11400.2809688001399915No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11210.276285986804325No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10830.26692036013299186No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10580.2607587636386939No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9050.22304979309358972No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8910.21959929905678283No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8570.21121952782453746No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC8340.2055508590497832No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8320.2050579313302394No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8220.20259329273252016No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG7750.1910094913232398No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7690.1895307081646083No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7360.18139740079213484No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7200.17745397903578408No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT7070.1742499488587491No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6940.17104591868171412No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6610.16291261130924065No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6410.15798333411380222No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6240.15379344849767954No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6210.15305405691836377No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5580.13752683375273267No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5490.13530865901478537No Hit
CGCACCATCTGGGAATCCGCTGCCGATATCG5390.13284402041706614No Hit
GGTGGCGAAGATGAGACCCGTCAGCCAGCAC4810.11854911655029465No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG4590.11312691163531235No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC4580.11288044777554045No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4470.11016934531804928No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT4390.1081976344398739No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4330.10671885128124238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)