FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404855
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57051.409146484543849No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC51531.2728013733311927No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG38260.9450297019920713No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT37100.9163774684763681No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA30340.7494041076434773No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT27560.6807375480110163No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG27070.6686344493707623No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG23980.5923108273332428No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC22710.5609415716738091No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA22310.5510614911511529No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC19900.4915340060021489No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA18350.45324869397685597No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17070.42163243630435593No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC15690.3875461585011918No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14920.3685270034950785No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG14030.3465438243321683No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG13210.32628965926072295No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC13020.3215966210124612No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC12790.3159155747119339No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12510.30899951834607453No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC12430.30702350224154323No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12280.3033184720455472No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12000.2964024156796878No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG10900.2692321942423831No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9820.24255597683121116No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9440.23316990033468774No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9260.2287238640994924No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8640.2134097392893752No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8630.21316273727630877No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8530.21069271714564472No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC8480.2094577070803127No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8020.19809561447925803No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7940.19611959837472676No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7890.19488458830939473No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7870.19439058428326192No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC7690.1899445480480666No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG7230.1785824554470119No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7170.17710044336861347No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7020.17339541317261736No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6510.1607983105062306No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6270.1548702621926369No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC6230.15388225414037124No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT6140.1516592360227736No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6060.14968321991824235No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG5990.14795420582677749No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC5970.14746020180064467No Hit
CTGCGTGTATTCGCTTGTTGCTGCGTTAAAC5860.14474317965691422No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5840.14424917563078138No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5640.13930913536945325No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5510.13609810919958998No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5360.1323930790035939No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5040.12448901458546888No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG5020.12399501055933605No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG4890.12078398438947278No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT4890.12078398438947278No Hit
ATCCACTCCCAGCGTGAACGTGACATGTTCC4820.11905497029800792No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC4710.11633794815427746No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC4670.11534994010201183No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4530.11189191191908214No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC4370.10793987971001964No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG4330.10695187165775401No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT4270.10546985957935558No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG4270.10546985957935558No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4250.10497585555322277No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG4240.10472885354015635No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4100.10127082535722667No Hit
ATGAGCGACGAAGGTCCGGGTACCGGCCCGG4080.10077682133109384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)