FastQCFastQC Report
Sun 21 May 2023
EGAF00007940974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529179
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG25470.4813116166741311No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19540.36925123634913704No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11200.21164861039459237No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9260.1749880475226719No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8620.16289384121440947No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG8390.15854748582237768No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT7310.13813851267718483No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC7290.13776056873005166No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG7280.13757159675648506No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7240.13681570886221864No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA6880.130012717813821No Hit
GTAGTTCGGGTCGATATCTGCGACACCTTCGGCGTCCAGCATTT6700.12661122228962224No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6610.12491047452752282No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA6400.12094206308262423No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT6030.11395010006066No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5830.110170660589328No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG5770.10903682874792839No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5380.10166692177883098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)