FastQCFastQC Report
Wed 31 May 2023
EGAF00007941004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456233
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT29070.6371744262251964No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC27550.6038581163572122No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA24090.5280196741577221No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA22650.49645685428278974No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA20470.4486742519721283No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20310.44516727198602474No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA18610.407905609633674No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18500.4054945608932278No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT14410.3158473849984547No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT14030.3075183075314587No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA12170.2667496651930044No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC11830.25929733272253436No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11740.257324656480351No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC10180.22313160161584103No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9300.20384321169227126No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9180.20121297670269359No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8070.1768833030490999No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7870.17249957806647043No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6970.1527728156446377No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6810.14926583565853413No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6690.14663560066895642No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA6540.14334780693198432No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6310.1383065232019604No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6210.13611466071064565No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6120.13414198446846237No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5940.13019663198409584No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5880.128881514489307No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5460.11967569202578505No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5260.11529196704315559No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5150.11288091830270937No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4980.1091547520674743No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG4960.10871637956921135No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG4690.10279835084266153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)