FastQCFastQC Report
Sun 21 May 2023
EGAF00007941015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476442
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC38770.8137401824356375No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT30940.6493969885106686No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG29800.6254696269430488No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22270.46742310711482193No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22070.46322532438366054No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19110.4010981399624718No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16450.3452676296380252No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16370.3435885165455606No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15160.3181919310220342No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG14840.3114754786521759No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14290.2999315761414821No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG13520.28377011262651064No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG13420.28167122126092997No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG12900.27075698615991034No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG11910.24997796164066138No Hit
CTGATGCGTATGAAGGAAGTATCTAGCGTTT11000.230878050213877No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10750.22563082179992527No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG10690.22437148698057685No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10640.22332204129778652No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10510.2205934825225316No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10430.21891436943006706No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG9970.20925946914839583No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9490.19918479059360844No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9430.19792545577426005No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9190.19288811649686635No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG8820.18512221844421778No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8150.17105964629482706No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7750.16266408083250428No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7030.14755206300032322No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6530.13705760617241972No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6520.13684771703586165No Hit
CTGTCCGACGAAGAGCGTGAAACCCTGGCAA6480.13600816048962938No Hit
AAGGAATCCGTTGCAAAAAGCGAAGCTGGCG6200.13013126466600342No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6110.12824226243698078No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5520.11585880338005465No Hit
TCCGAAAAATCTGAAAAATCTGACCGTAAAA5340.1120807989220094No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5250.11019179669298676No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5080.10662368137149957No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC4820.10116656382098976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)