FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007941251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427020
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64011.4989930214041496No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25300.592478104070067No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT14280.33441056624982435No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC11550.2704791344667697No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10320.24167486300407476No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8950.20959205657814622No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8810.20631352161491265No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8420.19718045993161915No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG7840.18359795794108003No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7790.18242705259706804No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7420.17376235305137933No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7120.16673692098730739No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6730.15760385930401385No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6650.15573041075359467No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6620.15502786754718748No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6470.15151515151515152No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6340.14847079762072035No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6210.14542644372628918No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5970.1398060980750316No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5960.1395719170062292No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5860.13723010631820523No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG5530.1295021310477261No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5500.1287995878413189No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4660.10912837806191747No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG4370.10233712706664794No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4370.10233712706664794No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4300.10069785958503115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)