FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007941253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454381
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT65631.4443825776165815No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA60571.3330222874636044No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA28700.6316285231996936No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27690.6094004810940599No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27310.6010374553513461No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22910.5042024204357136No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC21200.4665688045935019No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18250.40164531527506653No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18050.3972437227789014No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC17410.3831586267911731No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG17390.38271846754155653No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16690.36731289380497867No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC15820.34816596644666037No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC14830.3263780835906431No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC14730.3241772873425605No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14370.3162544208494633No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14160.31163274872849No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA13970.30745123585713313No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA13080.2878641492491984No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12360.272018416263004No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12330.2713581773885792No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11500.25309156852949394No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11460.25221125003026096No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11360.2500104537821784No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10470.23042336717424367No Hit
ATGTCCAACATGGACATCGATGGTATTAACA10210.22470129692922902No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9280.20423389182206123No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9180.20203309557397867No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8500.18706768108701727No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8120.17870465534430358No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6750.14855374674557253No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6740.1483336671207643No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6470.14239151725094137No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT6440.14173127837651664No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA6100.13424857113303593No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5830.128306421263213No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5650.12434498801666442No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5620.12368474914223966No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5620.12368474914223966No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5510.12126387326934886No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT5210.11466148452510118No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5200.11444140490029292No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5070.1115803697777856No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4910.10805909578085351No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4810.10585829953277096No Hit
GGTCAGGTGAAACCGGAAGTTAAACCAACCC4800.1056382199079627No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4720.10387758290949665No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG4690.10321734403507189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)