FastQCFastQC Report
Wed 31 May 2023
EGAF00007941257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493504
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA63801.292796005706134No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31160.6314031902476981No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA30290.6137741538062508No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA25360.5138762806380495No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24490.49624724419660227No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23650.4792261055634807No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT22060.44700752172221503No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17400.35258072882894564No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC16330.33089904033199324No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC15860.3213753080015562No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15130.3065831279989625No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13710.27780929840487617No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC13260.2686908312799896No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC13080.265043444430035No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12200.24721177538581246No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11780.2387012060692517No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC10320.20911684606406433No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA10030.2032405005835819No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9940.20141680715860458No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC9680.19614835948644793No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT9580.19412203345869536No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9390.1902720140059655No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8630.17487193619504604No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8590.17406140578394502No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8440.17102191674231618No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC8410.1704140189339904No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG8110.16433504085073272No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT7560.1531902476980936No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA7030.14245071975100507No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6970.1412349241343535No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG6150.12461905070678252No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT6140.12441641810400725No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG6050.12259272467902994No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6000.12157956166515367No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5900.11955323563740111No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5890.11935060303462584No Hit
GCTCCGACCTGCAACATCCGTGTTACCGTTT5530.11205582933471664No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC5480.11104266632084037No Hit
GAATGCGACAACTATCGCCAGGAATACGATA5340.10820580988198678No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5030.10192419919595383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)