FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007941299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502704
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT80311.597560393392533No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53861.0714058372322481No Hit
ATGTCCAACATGGACATCGATGGTATTAACA33980.6759444921862567No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA26070.5185954358827461No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17270.3435421241923677No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG14580.29003150959610424No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14300.284461631496865No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG14180.28207454088290523No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13250.2635745886247175No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12630.2512412871192591No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG12140.24149400044559027No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11910.23691874343550082No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11630.2313488653362615No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG11510.22896177472230178No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10630.21145644355326396No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10570.2102628982462841No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG10340.20568764123619468No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10200.20290270218657502No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG10060.2001177631369553No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC9930.19753174830516568No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9930.19753174830516568No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9590.1907683248989465No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9350.18599414367102707No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8550.17008020624462905No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8230.16371463127406982No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8150.16212323753143001No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8120.1615264648779401No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7490.14899423915465165No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7480.1487953149368217No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC7360.14640822432286196No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG7340.146010375887202No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7130.14183296731277253No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7060.1404404977879627No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG6980.1388491040453229No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6710.13347815016391357No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6420.12770934784684426No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6210.12353193927241478No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6070.12074700022279514No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6030.11995130335147523No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC5940.11816098539100543No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5940.11816098539100543No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5530.11000509245997646No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5410.10761800184601673No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5410.10761800184601673No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5260.10463413857856711No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5040.10025780578630766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)