FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007941493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465401
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68351.468625980605972No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31030.6667368570329673No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT30570.6568529074926783No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG29680.637729613816902No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG20010.4299518050025677No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19570.42049759239881307No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19510.41920838158921014No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19340.4155556176286686No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA18740.4026635095326396No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14670.31521204294790944No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14220.3055429618758877No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12500.26858558533393784No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10700.2299092610458508No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10090.21680228448155459No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10010.2150833367354174No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9200.19767899080577822No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8900.19123293675776373No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8730.1875801727972222No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA8380.18005977640787194No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8220.17662188091559752No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7550.16222569354169844No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7010.15062279625527233No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6790.14589568995339502No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6590.14159832058805202No Hit
GCGAAAACTGCGGCGGCCAAACCGGCTGCCA6520.14009424131018197No Hit
GCGGCAGCCGTTACCCAGGCACTGGGCAACG5960.12806160708722156No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5770.12397910619014571No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5550.1192519998882684No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5160.11087212962584954No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4950.10635989179223937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)