FastQCFastQC Report
Wed 31 May 2023
EGAF00007941497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448129
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64401.4370861961622656No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT56101.251871670880483No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28120.6274978856534613No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27240.6078606829729832No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC22260.49673196780391354No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18500.41282755635096147No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15090.33673339596410856No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13840.30883964215661114No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13470.3005830910295919No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13420.29946734087729204No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12020.26822633661289497No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG10260.2289519312519386No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9690.21623237951571983No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9490.21176937890652026No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9390.20953787860192044No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9090.20284337768812105No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG8750.19525627665248177No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8750.19525627665248177No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8670.19347107640880193No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8400.18744602558638251No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG8370.18677657549500257No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8030.17918947445936326No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7880.17584222400246358No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7760.17316442363694382No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7600.16959402314958416No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7510.16758567287544435No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7480.1669162227840644No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7300.1628995222357848No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6560.1463864199817463No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6520.14549381985990642No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC6280.14013821912886693No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5900.13165851797138772No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA5870.13098906788000778No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5390.12027786641792876No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC5340.11916211626562888No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5330.1189389662351689No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5210.11626116586964914No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5100.11380651553458937No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5030.11224446532136952No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC4990.11135186519952961No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4630.10331846410297034No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4600.1026490140115904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)