FastQCFastQC Report
Wed 31 May 2023
EGAF00007941501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490061
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA49911.0184446426057165No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29670.6054348336227531No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23230.47402262167362835No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22260.4542291673893658No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA20680.42198828309128866No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG18220.3717904505765609No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14080.2873111714664093No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA13520.27588402260126804No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13280.270986673087636No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG13260.2705785606281667No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12630.25772301815488274No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11730.23935795747876285No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10730.21895233450529628No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10370.2116063102348483No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10260.20936169170776697No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT10000.2040562297346657No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9810.20017916136970704No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9500.1938534182479324No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG9310.18997634988297377No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8520.17385590773393517No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC8290.16916261445003786No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC8250.16834638953109918No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7950.16222470263905922No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7650.15610301574701924No Hit
ATGTGCGAAGACACCATCACCTACGAATGTC7300.14896104770630594No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7290.1487569914765713No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6960.14202313589532733No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6920.14120691097638866No Hit
GTAGGTCCAACCCCTCACCAGACCCCGCAGG6780.13835012376010333No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6760.13794201130063402No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA6670.136105505233022No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6340.12937164965177805No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA6050.12345401898947274No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5680.11590393848929012No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5680.11590393848929012No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5640.11508771357035144No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5400.11019036405671948No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5230.10672140815123016No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5220.10651735192149549No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5210.10631329569176082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)