FastQCFastQC Report
Wed 31 May 2023
EGAF00007941502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454172
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT22700.49981064442545997No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC18400.4051328571554389No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16380.3606563152285918No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG15670.34502347128400696No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC14570.3208035722149318No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13080.2879966180213664No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG11240.24748333230582248No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11170.24594206600142676No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10580.23295139286437738No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10420.22942849845433008No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG10370.22832759395119032No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT9810.21599746351602475No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT9650.21247456910597748No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG9240.20344715218023127No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG9190.2023462476770915No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG8980.19772244876390443No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG8580.18891521273878617No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG7610.16755766537787448No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7520.16557603727222286No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG7390.16271368556405943No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG7080.1558880776445928No Hit
CTGGACGGAAACAGAACGACGGCTACGTTTGGAGTGGCGGGTAC6840.15060373602952185No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6690.14730102252010252No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6530.14377812811005522No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6440.1417965000044036No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT6190.13629197748870472No Hit
GGCGCGCGGTGGGGTCTGCATAGTCGCCGGTGCCCACTGACGCA6110.13453053028368106No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA6060.1334296257805413No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5960.13122781677426174No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG5580.12286094255039941No Hit
GTAAGCGCCCTGGGTGGTCTGCATGATAGCACCCGCTTTCGGGG5270.11603533463093278No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5170.11383352562465321No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA4910.10810882220832636No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT4880.10744827950644249No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4650.1023841187919995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)