FastQCFastQC Report
Wed 31 May 2023
EGAF00007941521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484563
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69981.4441878558618797No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA41630.8591246133113755No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT40840.8428212636953296No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29580.6104469387881453No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA19980.4123302852260697No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19580.40407542466098323No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA19460.4015989664914572No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18080.3731196975419089No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17150.35392714672808284No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15960.32936893654695054No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14980.30914452816248866No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC14760.30460435485169113No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12670.26147270839911424No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12220.25218599026339195No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG11740.2422801575852882No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10890.22473857888447943No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9720.2005931117316015No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT8930.18428976211555567No Hit
ATGGGTGCACAGGTTTCCACTCAGAAATCTG8750.18057507486126675No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8720.17995596031888528No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA8220.16963738461252714No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8090.16695455492887404No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7660.15808057982140608No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC7450.1537467780247357No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7450.1537467780247357No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA7350.15168306288346406No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7150.14755563260092083No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7000.1444600598890134No Hit
ACCACTCCGACGAAAAGCCACTTCGCAAACC6870.1417772302053603No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG6550.13517334175329113No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6120.12629936664582314No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5500.1135043327699391No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5460.11267884671343044No Hit
GTTACCCTGGATGCAGGTAACCGTTTCTCTA5450.11247247519930328No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5180.10690044431786992No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA5140.10607495826136126No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5120.10566221523310695No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG5050.1042176146342168No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5050.1042176146342168No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4990.10297938554945385No Hit
ATCCCGCTGACCACTACCCCAACCAAATCCC4980.10277301403532667No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4970.10256664252119951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)