FastQCFastQC Report
Sun 21 May 2023
EGAF00007941556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526083
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25850.49136733177084224No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24480.46532581360735853No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT23760.45163976026596564No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC18290.3476637716862168No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC15880.3018535098073878No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA13390.25452257533507067No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT13290.2526217345932106No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12050.22905130939414503No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG10760.20453046382414944No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10730.20396021160159136No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT10640.20224945493391727No Hit
AACCAGCGGTACCAGGGACAGCTGCAGGCGTGGCGGACCGGTTT10080.19160474677950057No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT9980.18970390603764045No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG9560.18172037492182794No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9020.17145583491578326No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG8840.16803432158043502No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8710.16556322861601686No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG8690.16518306046764483No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT8560.1627119675032267No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT8170.1552986886099722No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT7910.15035650268113585No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC7830.14883583008764778No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG7220.137240701562301No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA7090.13476960859788284No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7010.13324893600439475No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG6960.13229851563346468No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6910.1313480952625346No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6780.12887700229811647No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6640.12621582525951228No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG6450.12260422784997804No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6450.12260422784997804No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6230.11842237821788577No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6010.11424052858579349No Hit
CACCAGCGGAACCAGGGACAGCTGCAGACGCGGAGGACCGGTTT5780.10986859487951521No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5760.10948842673114319No Hit
CCACTGCGGCGGCAGATGCGGAGCACGCGGGTCCCAAGGGCCCT5510.10473632487649287No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT5380.10226523191207472No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT5370.1020751478378887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)