FastQCFastQC Report
Wed 31 May 2023
EGAF00007941565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470452
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46260.9833096681489292No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA37370.7943424621427904No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT25520.5424570413134602No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA18630.39600214262028854No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18020.3830358888898336No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16820.3575285045020533No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16120.34264919694251483No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC15990.339885896967172No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15770.33520954316274565No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14690.3122528972137434No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13860.29461028967886205No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13710.2914218666303895No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC13240.28143147441184224No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG12890.27399182063207295No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12350.26251349765757187No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG11720.24912212085398724No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11660.24784675163459824No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC11510.24465832858612568No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10760.228716213343763No Hit
GCCGGTCAGCCGAGCGAACCGTGGCCGGATC10140.21553739807674321No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC9500.20193345973659374No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8950.1902425752255278No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7950.16898642156904425No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7870.16728592927652555No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7820.1662231215937014No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7630.1621844523989695No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7130.15155637557072774No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7040.1496433217416442No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6890.14645489869317166No Hit
ATGTCCAACATGGACATCGATGGTATTAACA6760.1436915987178288No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6340.13476401418210573No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6150.13072534498737384No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5890.12519874503668812No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5770.12264800659791009No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5740.12201032198821558No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5670.12052239123226174No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5540.11775909125691887No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5540.11775909125691887No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5440.11563347589127052No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5400.11478322974501118No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5370.11414554513531666No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5340.11350786052562217No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC5200.11053199901371448No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5080.10798126057493644No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4770.10139185294142654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)