FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007941593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007941593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520314
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC43910.843913483012181No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG40710.7824121588117945No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT30720.5904127123237122No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT27640.5312176877808401No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA26120.5020045587856564No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24160.46433499771291953No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC18790.3611280880391456No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18630.35805302182912624No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG18380.35324823087597107No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17970.3453683737127965No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17570.33768070818774815No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16810.3230741436901563No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG16240.31211922031696243No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG15130.2907859484849533No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15040.28905622374181744No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14740.2832904745980312No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG14390.2765637672636139No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13240.2544617288790999No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC12840.2467740633540516No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC11870.22813147445580936No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11300.21717655108261547No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11190.2150624430632272No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10960.21064203538632442No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10900.20948888555756714No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10900.20948888555756714No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10650.20468409460441198No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10590.2035309447756547No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10230.19661204580311123No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9660.18565712242991733No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9560.18373520604865523No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8230.15817371817786952No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7590.1458734533377922No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7460.14337496204215147No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT7270.1397233209177535No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7260.1395311292796273No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7090.13626387143148175No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC7000.1345341466883459No Hit
GCGAAAACTGCGGCGGCCAAACCGGCTGCCA6880.13222784703083137No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6790.13049812228769553No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC5670.10897265881756016No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5340.10263033475939529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)