FastQCFastQC Report
Wed 31 May 2023
EGAF00007942155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556239
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA83131.4945014642986199No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA73751.3258689160594637No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT36870.6628445686116938No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26500.47641391560102764No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA25040.45016620553395215No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC24010.4316489854181386No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT23510.4226600436143456No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT18010.3237816837726229No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15360.2761402922125202No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT14460.25996019696569284No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14300.2570837355884791No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13440.24162275568595515No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG13220.23766762129228625No Hit
TTCCTCACCGGCAGCTTTTGCCTGTTCTTCAATGTTAGCAACAA11560.20782433450369356No Hit
GTCGGAAATCTGTTTCTCTTCCTTAACTTTATCCAGTTCCGCGG10840.19488025830623168No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT10780.1938015852897765No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG10170.1828350762891491No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10150.18247551861699737No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT10070.1810372879283905No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8810.1583851545828322No Hit
TGGAATGGTTTTGCAGATGGATTTGCACAGCTGGTTGTTACCGC7920.1423848381720807No Hit
TACGGTATCAGGGGATTCCGGCTGCACCTCGGTTTCGCCTTTGT7760.13950837679486697No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7570.13609257890942564No Hit
GCCTTGACGAGATGCGTCGGAAATCTGTTTTTCTTCTTTGACCT7440.13375545404043945No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG7060.1269238582695568No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT6850.12314850271196376No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6740.1211709355151293No Hit
TGGGATAACATCGGTCGGAATGTAGGACAGAGATTCCTCCGCGT6720.12081137784297757No Hit
TTCTTCAGTGTGGTCCTCTTCAGATTTTTTCTTGTCCTCGCTCT6600.11865403181006726No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG6550.11775513762968795No Hit
TCAACCAATGTGTGGGGTAGTTGCGACGGTGTCTACCAGGATGC6420.1154180127607018No Hit
GGTCGGTTTTTTCTTCGGTTTGTTGGACGGAATGGTCTTGCAGA6350.11415956090817077No Hit
GTCAACCTGGTTGTCGTTCGCGATTTTCTCGGTGATAGCTTCCA6340.1139797820720949No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG6240.1121819937113363No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA5830.10481106143222607No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5780.10391216725184678No Hit
TTCCAGGTTCGCGCGCTCGCCAGAATCGTGCGGTTTCACAATCT5750.10337283074361919No Hit
GATTTCGTTAACGATATCGCTGTCCTTCAGCTGATAGTTCGGGT5730.10301327307146747No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5710.10265371539931575No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT5680.1021143788910882No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5660.10175482121893646No Hit
GTATACGTTACCGCTAACGGTGCCACCGGTGGACGGCAGAACTT5570.10013681169425373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)