FastQCFastQC Report
Sun 21 May 2023
EGAF00007942156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556239
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT183763.303615891729994No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC38790.6973621051382589No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT37010.6653614723167559No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA26890.48342529020798614No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA24750.4449526192877522No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17070.3068824731814921No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14880.2675109080808789No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14630.26301643717898243No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14540.2613984276542997No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA14460.25996019696569284No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA11670.209801901700528No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11660.20962212286445214No Hit
AAACAAGATTTCGGTGCGCTGAAACAGGAGC11430.20548720963470737No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11130.20009384455243162No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT10310.1853519799942111No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC10040.18049795142016292No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9920.1783406053872526No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9810.17636303819041815No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG8710.15658736622207362No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8170.1468793090739772No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8070.14508152071321861No Hit
AACCTGACCGCGGAACTGGATAAAGTTAAAG7890.14184550166385312No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7700.1384297037784118No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT7570.13609257890942564No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7340.13195766567968087No Hit
ACCACCACTTCTACTCAGACGAATAAACCGA7090.12746319477778437No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7060.1269238582695568No Hit
GTGCGCCAGGAAAACCTGCAGATTCTGATGA7060.1269238582695568No Hit
CCGAGCACTAAACCGCGTCCGAAAAACCCTC7040.1265643005974051No Hit
ACCACCAACGAACAGCCGAAACCAGAATCTT7000.12584518525310162No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6970.12530584874487405No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA6870.12350806038411545No Hit
CCGAAAGACCAGTTTCTGGAAGCAATCACCG6840.12296872387588789No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG6630.11919336831829484No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC6470.11631690694108107No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6310.11344044556386734No Hit
CAGATCGTGATCAAAATTACCGATCAGGGTT6220.11182243603918461No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6120.11002464767842599No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6120.11002464767842599No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6000.1078673016455157No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5960.10714818630121224No Hit
GTTGGCAAAAGCATTGAAGATGCTCGTGAAG5940.10678862862906052No Hit
GTCGTTGTATCTGCAAAAAAATTCGCATCTA5820.1046312825961502No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5660.10175482121893646No Hit
ATCGTTTACGGTCCGCGTGTAGGTGACCCAG5630.10121548471070888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)