FastQCFastQC Report
Sun 21 May 2023
EGAF00007942158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500411
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT52971.0585298884317091No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16770.3351245276382813No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA9990.199635899290783No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9810.1960388560603184No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9490.18964411253949254No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9260.18504789063389893No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT7820.15627154479018246No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT7760.1550725303800276No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7190.1436818934835565No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC5850.11690390499009813No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5760.11510538337486587No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA5370.10731178970885932No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC5310.10611277529870447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)