FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007942166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495894
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT64021.291001706009752No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT31280.630779965073181No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18010.3631824543148334No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16010.32285125450197016No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14920.30087075060395974No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14860.29966081460957383No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC13590.2740505027284057No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12740.2569097428079388No Hit
ATGTGCGAAGACACCATCACCTACGAATGTC11580.23351764691647814No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10550.21274707901285358No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10070.20306759105776637No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9950.2006477190689946No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9720.19600963109051533No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9340.1883467031260713No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8500.17140759920466875No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8330.16797944722057537No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8100.1633413592420961No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8010.16152645525051723No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7800.15729167927016663No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7560.15245193529262302No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7390.14902378330852964No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7370.14862047131040101No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7230.14579728732350059No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7040.14196582334127858No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7040.14196582334127858No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6880.13873932735624953No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6620.1334962713805773No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA6440.1298664633974196No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6170.12442175142268308No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG6010.12119525543765403No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5890.11877538344888222No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5830.11756544745449632No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5620.11333067147414569No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5590.11272570347695274No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5280.10647436750595893No Hit
GCCAGCGCACAGGGTACGGCGGACGATACCA5080.1024412475246726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)