FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007942167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490201
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA43820.8939190250529885No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA39880.8135438320199264No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT25360.5173388059183887No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21930.4473675084302154No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT21440.43737160878904774No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG18530.3780082047976238No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17360.3541404444299379No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG15340.3129328581541041No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15010.3062009257427055No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13670.27886520019339006No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12570.2564254254887281No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC11560.2358216323508112No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA11370.2319456712654605No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11320.2309256815061577No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG10140.20685392318661122No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT9660.19706202149730417No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9580.1954300378824197No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9070.18502614233753095No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT8870.18094618330031967No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8370.1707462857072915No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8270.16870630618868587No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7810.15932240040309995No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7580.154630447510307No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7480.15259046799170137No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7370.15034649052123517No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7320.14932650076193235No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7180.14647052943588446No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG7130.14545053967658164No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7090.1446345478691394No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6850.13973859702448588No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT6770.13810661340960137No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA6670.13606663389099574No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6470.13198667485378446No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT6380.1301506932870394No Hit
GCCGCGGGTAATCTGGATCAGACGGTCAGAGTAGCCGCAGGCTT6240.1272947219609915No Hit
GTTCAGACCGAACTCTTCTGCAAATTTCTTGGCACCTTCCTGCA6060.12362275882750137No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC5920.12076678750145349No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5690.11607483460866053No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5590.1140348550900549No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT5430.11077088786028588No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5390.10995489605284361No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5360.10934290219726193No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT5300.10811891448609856No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5110.10424295340074785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)