FastQCFastQC Report
Sun 21 May 2023
EGAF00007942168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490201
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT101552.0715992011440205No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30250.617093804378204No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25730.5248867301372294No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22250.4538954428897534No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC20410.4163598197474097No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18810.3837201474497196No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18620.3798441863643689No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18080.36882829696389846No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15520.31660482128759426No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15300.3121168663466619No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14410.29396104863107175No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA14060.286821120315952No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13520.2758052309154816No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12490.25479344187384356No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11270.22990569174685485No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10660.21746181668336048No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9790.1997139948714915No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9620.1962460296898619No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8800.1795181976372957No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8490.17319426112961825No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8180.1668703246219408No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7970.16258636763286896No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7680.15667042702891262No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7660.1562624311251915No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7620.15544643931774926No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7600.15503844341402812No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7560.15422245160658588No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7370.15034649052123517No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7350.14993849461751405No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7220.14728652124332672No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7000.14279856630239432No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG6890.14055458883192812No Hit
GACGCAGCTTCCTCTGGTGCGAGCCGTCTGG6850.13973859702448588No Hit
CTGCGTTTCAAAACCGGCCGTAACGCTGCGT6270.1279067158165732No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6190.1262747322016887No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6190.1262747322016887No Hit
ATGGGTGCACAGGTTTCCACTCAGAAATCTG6000.12239877111633798No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5890.1201547936458718No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5840.11913480388656898No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5800.11831881207912673No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5600.11423885304191546No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5320.1085269103898197No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5110.10424295340074785No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5070.10342696159330561No Hit
AACCTGACCGCGGAACTGGATAAAGTTAAAG5030.10261096978586336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)