FastQCFastQC Report
Wed 31 May 2023
EGAF00007942170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463696
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT92932.0041147648459336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44810.9663658948966564No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28350.6113919464476726No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24680.5322452641385735No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20100.4334736551533764No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC19290.41600531382629996No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17200.3709326800317449No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG17190.37071702149684277No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16800.36230633863565787No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14620.3152927780269832No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14160.30537248542148304No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13090.2822970221869501No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12770.2753959490700804No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12260.26439736379006934No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12020.2592215589524171No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10580.22816672992650358No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10120.21824643732100343No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8590.1852506814809703No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8440.18201580345743762No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8130.17533038887547014No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7920.17080155964252441No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7790.1679979986887961No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7340.15829336461819812No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7210.15548980366446982No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7190.15505848659466548No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7140.15398019392015458No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6920.14923570615230666No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6900.14880438908250232No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6790.1464321451985784No Hit
CTGCGTTTCAAAACCGGCCGTAACGCTGCGT6650.1434129257099479No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6530.14082502329112176No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6460.13931541354680654No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6290.13564921845346953No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG6140.13241434042993686No Hit
GACGCAGCTTCCTCTGGTGCGAGCCGTCTGG6100.13155170629032814No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA6010.12961077947620855No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5900.12723853559228462No Hit
ATGGGTGCACAGGTTTCCACTCAGAAATCTG5630.12141575514992581No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5610.12098443808012146No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5560.11990614540561058No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5390.11623995031227356No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5170.11149546254442565No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4960.10696663331147993No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC4680.10092819433421896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)