FastQCFastQC Report
Wed 31 May 2023
EGAF00007942171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437053
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA31070.710897762971539No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA28410.650035579208929No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20750.4747707943887812No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17840.4081884805732943No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17490.4001802984992667No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA16300.3729524794475727No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG13040.29836198355805815No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12980.2969891523453677No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT12400.28371845062269335No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11490.2628971772302215No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11120.2544313847519637No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG9980.22834759171084512No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9780.22377148766854366No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG9480.21690733160509137No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG9280.21233122756278985No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG9120.20867034432894868No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9030.206611097509913No Hit
CCAGGCAGAACCGTGGTGGTTACGGGATGCGCTACCAGAATGGC8420.1926539801808934No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8310.19013712295762758No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8310.19013712295762758No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8030.18373057729840547No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG7730.1768664212349532No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG7660.17526478482014768No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT7240.16565496633131452No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT7050.16130766749112807No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6830.1562739530445964No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6290.14391847213038236No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6160.14094400450288638No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6140.1404863940986562No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG6060.13865595248173562No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5860.13407984843943413No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG5770.13202060162039844No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5550.12698688717386677No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5240.11989392590829945No Hit
ACCCTGGTGCAGACCCAGGACTGGAGACAGTTCCGGCATGGACG5190.11874989989772407No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5130.11737706868503361No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5040.11531782186599794No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4850.11097052302581152No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT4840.11074171782369643No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT4780.109368886611006No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG4550.10410636696235925No Hit
GTGCAGGGCCAGCATGATAGCCTGCAGTTCGGTTTTGGAAATTG4390.10044548372851805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)