FastQCFastQC Report
Wed 31 May 2023
EGAF00007942172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437053
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT70121.6043820772309079No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43230.9891248887434704No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27930.6390529295074052No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18430.4216879874980838No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17840.4081884805732943No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17350.39697702566965565No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14660.33542842630070036No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13720.3139207373018833No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12780.29241304830306625No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12640.28920977547345517No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11980.27410863213386016No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11470.26243956682599134No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10350.23681338418910292No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9540.21828016281778181No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG9160.20958556513740897No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8970.20523826629722255No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8640.19768769462742505No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8640.19768769462742505No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG8570.19608605821261954No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC8430.19288278538300846No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8280.18945070735128233No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7610.17412075880957228No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG7530.1722903171926517No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7310.16725660274612003No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG7130.16313810910804868No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6650.15215545940652506No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6500.14872338137479896No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6300.1441472773324974No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6300.1441472773324974No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6060.13865595248173562No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5780.13224940682251352No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5720.13087657560982305No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5710.130647770407708No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5500.1258428611632914No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5360.12263958833368035No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5230.11966512070618437No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5100.1166906530786884No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5050.11554662706811303No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5020.11486021146176778No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5000.11440260105753765No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4820.11028410741946629No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC4720.10799605539831554No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT4690.10730963979197032No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4510.10319114615389895No Hit
GTTATTTCTCTGACTCGTCGCACCCGTCAGA4500.10296234095178389No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA4490.1027335357496688No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG4480.10250473054755373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)