FastQCFastQC Report
Wed 31 May 2023
EGAF00007942173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466836
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA33010.7071005663659187No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA30630.6561190653677094No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21650.463760292693794No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19660.4211329032036947No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17720.37957655365053256No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA17000.3641535785586373No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG14230.30481796605231815No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13760.29475019064510877No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13010.27868459159105125No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12920.27675671970456434No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11510.24655339348293617No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA10690.22898833851716663No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG10530.22556101071896767No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG10410.22299051487031846No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG10290.2204200190216693No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG10070.21570744329914576No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9920.21249432348833422No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8910.1908593167622034No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8910.1908593167622034No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8620.18464728512796785No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8120.17393688575859617No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG8050.17243742984688412No Hit
CCAGGCAGAACCGTGGTGGTTACGGGATGCGCTACCAGAATGGC8020.17179480588472182No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT7440.15937074261625067No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT7400.15851391066670092No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7380.15808549469192607No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6860.14694667934777952No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG6540.14009202375138166No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6530.13987781576399422No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6360.13623627997840784No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG6360.13623627997840784No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6330.13559365601624554No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6200.13280895218020888No Hit
ACCCTGGTGCAGACCCAGGACTGGAGACAGTTCCGGCATGGACG5920.12681112853336077No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5710.12231276079822465No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5570.11931384897480056No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5240.11224498539101527No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5150.11031711350452836No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT5070.10860344960542888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)