FastQCFastQC Report
Sun 21 May 2023
EGAF00007942174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466836
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT72391.5506516206976326No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30630.6561190653677094No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19820.4245602310018936No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19390.41534928754423395No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18020.38600279327215553No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15640.3350212922739463No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15130.3240966849171872No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14220.30460375806493073No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13400.28703870309916113No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12540.26861681618384187No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11740.25148017719284715No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11220.2403413618487006No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10700.22920254650455407No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG10420.22320472285770593No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG10000.21420798738743368No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA9970.2135653634252714No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9230.19771397235860128No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9040.19364402059824007No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8980.19235877267391546No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8910.1908593167622034No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8550.1831478292162558No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC8140.17436530173337103No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG7920.1696527260108475No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7760.16622539821264853No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG7550.16172703047751244No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7040.15080242312075334No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6710.143733559536968No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6700.14351935154958056No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6520.13966360777660677No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6510.13944939978921936No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6510.13944939978921936No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6490.13902098381444447No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6470.1385925678396696No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5840.12509746463426127No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5800.12424063268471155No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5790.12402642469732411No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5550.1188854330000257No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5540.11867122501263827No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5490.1176001850757011No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5400.11567231318921421No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC5290.11331602532795243No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5160.11053132149191579No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5120.10967448954236605No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5070.10860344960542888No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4960.10624716174416711No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4950.10603295375677968No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4720.10110617004686871No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)