FastQCFastQC Report
Wed 31 May 2023
EGAF00007942188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389604
Sequences flagged as poor quality0
Sequence length31
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55221.4173365776532068No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA48391.2420303692980563No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA45591.170162523998727No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC37030.9504522540836338No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG29290.7517889960062012No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27490.705588238313775No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG27320.7012248334206014No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23820.6113900267964395No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23400.60060985000154No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC22950.5890596605784335No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19550.5017915627149618No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC18520.47535446247985136No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA18050.46329093130460675No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17260.44301393209515305No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT16150.4145234648514903No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15530.3986098705352101No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC15500.397839857907003No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14950.38372295972320614No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA14410.3698627324154783No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13770.35343579634706007No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA12990.33341546801367544No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11830.30364164638966745No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11330.29080810258621576No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG11040.28336464718021376No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG10010.2569275469451032No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT9850.2528208129279987No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9670.24820073715875607No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC9440.2422973070091683No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG7760.19917659982957056No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7620.19558320756460404No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7220.1853163725218427No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7200.18480303076970464No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7180.18428968901756654No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCGC7180.18428968901756654No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7040.18069629675260007No Hit
CCGTTCGCCTCTGATTACTCCCAGGGTGCGT6830.17530620835515034No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6630.17017279083376968No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6300.16170265192349154No Hit
ATCGTCACCCCACCGTCCGCGCGTCCGCGTA6150.15785258878245603No Hit
TTCTCCCAGGTTGCTGACGTAGTCCGTGCAC6140.157595917906387No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5950.15271917126107534No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5670.1455323867311424No Hit
GGCCGCCCTTCCCTGAAACTGCAGGCTGCCC5570.14296567797045204No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5290.1357788934405191No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC5150.1321855011755526No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5070.13013213416700034No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC4990.12807876715844804No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4900.12576872927382676No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC4880.1252553875216887No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4860.12474204576955063No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG4820.12371536226527449No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4790.12294534963706738No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4790.12294534963706738No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC4740.12166199525672221No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4680.12012197000030801No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG4600.11806860299175574No Hit
GTACTGCCGACCCCGATGCAGCTGGCACTGC4510.11575856510713442No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG4460.11447521072678925No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA4320.11088181846182277No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4320.11088181846182277No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4300.11036847670968472No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4120.10574840094044208No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG4090.104978388312235No Hit
GGTTACCCAGGTCCGCGCGGTGTCAAAGGTG4080.10472171743616594No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA4000.10266835042761367No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA3950.10138499604726851No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT3920.1006149834190614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)