FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007942189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425489
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA74771.7572722209034781No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA69231.6270690899177183No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT33280.782158880723121No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT32730.769232577105401No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC28980.6810986888027657No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG28260.6641769822486597No Hit
TTTAGCTTCACGACCACGAGAACGAGAGCGGGCGCGACGGGTTG23540.5532457948384094No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG22160.5208125239430397No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC19390.45571095845015963No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA18960.44560493925812417No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT18680.4390242755981941No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT16400.3854388715101918No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG16280.38261858708450747No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC15830.37204252048819125No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT15180.35676597984906777No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT14960.3515954584019798No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC14570.34242953401850573No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG13720.3224525193365751No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11890.2794431818448891No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10980.25805602495011626No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10880.25570578792871257No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT10620.24959517167306325No Hit
TTTAGTGCCGTTGACTGCAATTTCAACACGGCGGTCCGGCGCCA9710.22820801477829042No Hit
AATCAGCGGGTTGAATGCCTGTTTTACCTTTTTCGGGTGTTTCT9110.21410659264986873No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC8110.19060422243583267No Hit
GATACGGTGGGCGGTCAGATCGTTCTCCCACGCGAAGTCATCCA7690.1807332269459375No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT7620.17908806103095498No Hit
ACGACATTTACGGGAAGCGACACGGTTTTTGTAACGCTTAATCT7380.1734474921795863No Hit
AGTACCCGGACGGAAGTTGGACTGGAACAGCAGGGTGTCGGTAG7070.16616175741323513No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7030.1652216626046737No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6770.1591110463490243No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6770.1591110463490243No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6670.15676080932762068No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT6580.15464559600835745No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA6500.15276540639123454No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6170.14500962422060265No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6080.1428944109013394No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5910.13889900796495327No Hit
GATGAGCGGGTTGAACGCCTGTTTAACCTTTTTAGGGTGTTTCT5880.13819393685853218No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT5650.1327883917093039No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC5380.12644275175151415No Hit
TTTCGGGTGTTTCTGCTTCTGACGGGCTTCGCTGGAGGTACGTT5220.12268237251726838No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5100.11986208809158404No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5080.11939204068730332No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA4790.11257635332523284No Hit
GATCAGTGGGTTGAACGCCTGTTTAACTTTTTTTGGGTGTTTCT4690.11022611630382925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)