FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007942190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425489
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT166253.907269048083499No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA68871.6186082366406653No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT32950.774403098552489No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA30450.7156471730173988No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA28300.6651170770572212No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA24720.5809785916909721No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC22920.5386743253057071No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA22510.5290383535179524No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC19850.4665220487486163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19190.4510104844073525No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG18350.4312684934275622No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA17030.4002453647450345No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG16810.3950748432979466No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG15590.36640195163682254No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13280.3121114764423992No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12490.2935446039733107No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11370.2672219493335903No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11050.25970119086509874No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10710.25171038499232645No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10250.2408992946938699No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA9850.23149834660825547No Hit
GCCCGCCCGGAAACTCCATCCCCGGCAATCC8470.19906507571288568No Hit
ACCGATGGTGACGGTACTACTGATACCCTGC8080.18989915132941157No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT8020.1884890091165694No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7670.18026317954165677No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7430.17462261069028812No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG6800.15981611745544538No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6780.15934607005116466No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6590.1548806197104978No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG6580.15464559600835745No Hit
AACCCACTGGGCCGTTTTCGTCCGGCCGAAA6480.15229535898695382No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6290.14782990864628698No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6230.14641976643344481No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6140.14430455311418156No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT6080.1428944109013394No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6000.1410142212842165No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG5820.13678379464569002No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5390.1266777754536545No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5260.12362246732582981No Hit
GCACGCCCGGAAACCCCGTCCCCGGCAATTC5140.12080218290014548No Hit
CCGCCTCGTACCCCAACCTGGGAATCTCCGG4820.11328142443165393No Hit
CAGGGCCAGCAGCTGGCCGATATCGGTGTGG4540.10670076077172383No Hit
GACGCCGCGTCTTCTTCTTCCGCGGGTCAGT4400.10341042894175878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)