FastQCFastQC Report
Wed 8 May 2024
EGAF00007942192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422686
Total Bases13.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25820.6108553394245373No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA19640.4646475161230796No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC18340.43389182513733604No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17280.4088141078720374No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16750.39627524923938806No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16300.38562904851355384No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG15850.3749828477877195No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG14820.3506148772374765No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14490.3428076633718647No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13600.32175184415854796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13260.31370804805458424No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13240.31323488357788054No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG11470.2713598273895989No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10420.2465186923626522No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10060.23800173178198472No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC9640.22806527777120603No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9560.22617261986439108No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8800.20819236974964866No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC8740.20677287631953745No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8550.20227781379085183No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC8240.19494376440194378No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8180.1935242709718325No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8060.19068528411161004No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7950.18808287948973942No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7410.17530743861873824No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7400.17507085638038639No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6970.16489782013125584No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6800.16087592207927398No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6580.15567111283553275No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6450.15259554373695838No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6420.1518857970219028No Hit
CTGCCGGTTACCGTCACCATCGCCCCGATGT6360.15046630359179153No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6190.1464444055398097No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA6040.1428956719645316No Hit
TGTGAACGTTTCGGCGCCGCCGTCACTGACA5810.1374542804824385No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5680.13437871138386415No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5440.1287007376634192No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5400.12775440871001167No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5120.12113010603615924No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5060.11971061260604798No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4980.11781795469923301No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4860.11497896783901052No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC4690.11095706978702868No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG4510.10669858949669495No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4320.10220352696800936No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4300.1017303624913056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)