FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007942195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423798
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA43851.0346910556444344No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA39000.9202497416221879No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT31910.7529530578247184No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC25950.6123200203870711No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19680.4643721773108887No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT18370.4334612244512716No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC17710.4178877672853577No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC15250.35984124512149657No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC14120.3331775987616742No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG13270.3131208736237547No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG13020.30722183681848425No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG11370.2682881939036994No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA10760.25389454409883955No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG9790.23100628129439027No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG9700.2288826280444929No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC9020.21283724793415731No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG8810.20788205701773016No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC8350.19702782929603255No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8070.19042090807412965No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6860.16186956993662074No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6010.14181284479870127No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5960.14063303743764718No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG5830.13756553829890655No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5830.13756553829890655No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT5670.13379015474353348No Hit
GGATGCGGACGCAGTGGTAGCACGAGACTGACGCGGGGCGGTAG5660.13355419327132265No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5620.1326103473824794No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5560.13119457854921449No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT5280.1245876573273116No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5230.12340784996625752No Hit
CGGTTTGTCAACCGCGGTGGCGTCATCGTCGCTGCAATAGCTTG4880.1151491984388789No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4850.11444131402224644No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG4810.11349746813340317No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4760.11231766077234909No Hit
GTTGTAGAAAACGGTAGGGCTACGCGGTTCCGGGGAATCTGGGT4700.11090189193908419No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG4540.10712650838371111No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT4310.10169939452286231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)