FastQCFastQC Report
Wed 31 May 2023
EGAF00007942196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423798
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT99582.34970434027532No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA65161.5375249529256863No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA29830.7038730716048683No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23630.5575769588341616No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19750.4660239076163644No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA18450.43534891622895816No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC15200.35866143776044246No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA13600.32090760220671166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13240.3124129892071223No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC11700.2760749224866564No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC11480.2708837700980184No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC11200.2642768488761155No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT10110.23855704840513642No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9260.21850032326721694No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA8270.195140137518346No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7700.18169033360232942No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6400.15101534221492316No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6360.15007149632607988No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6210.14653207424291761No Hit
TCTAACAGCGTAAACGGTTCTTCCCAGGCCG5910.1394532300765931No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5860.138273422715539No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5810.13709361535448492No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5720.13496996210458756No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5610.13237438591026857No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC5550.13095861707700368No Hit
ACCACCCTGCCGCCGACTCCGCTGCTGACCG5320.12553150321615486No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5200.12269996554962505No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5120.12081227377193852No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5110.12057631229972769No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5000.11798073610540871No Hit
AAATCCCCGTCTGCAGAAGCGTGCGGCTACT4820.113733429605614No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4720.11137381488350581No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG4430.10453093218939212No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4400.10382304777275966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)