FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007942200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412970
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT86052.083686466329273No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25240.6111824103445771No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21890.530062716420079No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19370.4690413347216505No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC15620.37823570719422717No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT13720.3322275225803327No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12490.30244327675133786No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG11650.28210281618519506No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11050.26757391578080736No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9160.221807879506986No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8940.21648061602537716No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC8070.19541371043901493No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7780.1883914085768942No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC7570.1833062934353585No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6760.16369227788943508No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6720.16272368452914257No Hit
GTTACCATGACCTTCAAGCTGGGCCCTCGTA6310.15279560258614427No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6160.14916337748504732No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6010.1455311523839504No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5910.1431096689832191No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5830.14117248226263407No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5430.13148654865970894No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG5060.12252706007700317No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4940.11962127999612562No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC4540.10993534639320048No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4540.10993534639320048No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4420.10702956631232292No Hit
GGTCGCTGGAACCCGCAGCCGGGCGTGTACC4280.10363948955129912No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC4260.10315519287115286No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA4170.1009758578104947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)