FastQCFastQC Report
Sun 21 May 2023
EGAF00007942210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385941
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT62501.6194185121560032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51151.325332110348473No Hit
ATGTCCAACATGGACATCGATGGTATTAACA15460.4005793631669089No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC13200.34202118976734786No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12310.31896067015424634No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12240.31714692142063167No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC10870.2816492676341721No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10000.2591069619449605No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC8460.21920448980543658No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG8330.2158360993001521No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7770.20132610943123433No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7670.1987350398117847No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC7460.19329379361094054No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA6230.1614236372917104No Hit
GGCCGCTCCGACGCGTCCCATGGCCAGTCTG5990.15520507020503135No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5920.15339132147141663No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5720.14820918223251742No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT5450.14121329426000348No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5400.13991775945027868No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5150.13344008540165467No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4740.12281669996191127No Hit
GGTCAGAAAGGTGGCGCGCAGAAGTCCCAGA4510.11685723983717719No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG4380.11348884933189271No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC4370.11322974236994773No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT4340.11245242148411286No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4300.11141599363633302No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA4200.10882492401688341No Hit
TCTGGCTACGAAATTGACCACATCGACCTGG4040.10467921262576403No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4030.1044201056638191No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4010.10390189173992916No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA3920.10156992908242453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)