FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007942213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427324
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG27920.6533684043021221No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG25210.5899504825378402No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA23340.5461897763757712No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA22030.5155338806151772No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG21190.49587666501296446No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20960.4904943321695014No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG20380.4769214928251163No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG20250.47387930469620243No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT17670.4135035710608344No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG16650.3896340949724331No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG16460.3851878200147897No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG16280.38097555952860124No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG15410.36061630051202365No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG14910.34891557693927794No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT14010.3278542745083356No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA13320.3117072759779465No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG12850.3007085958195655No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG12640.2957942919190123No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG11260.263500294858234No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG10720.25086351339966867No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC10400.24337505031311138No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9190.21505929926706668No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT8850.20710280723759958No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8430.19727419943649316No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG8410.19680617049358334No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG8020.18767960610684165No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG7710.1804251574917393No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG7600.17785099830573523No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG7570.17714895489137047No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7520.1759788825340959No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG7170.1677883760331739No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG7070.16544823131862474No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG6990.16357611554698542No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC6960.16287407213262067No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG6810.15936385506079698No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA6740.15772575376061254No Hit
CGGGCACTGCGGAACGGTCAGGTACAGGCCGCGACGTTTTTCCG6680.15632166693188307No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG6640.1553856090460634No Hit
CGGGGTCAGGCGAGGCTGGGACAGCGGAGACAGAATGGTCGGCG6460.15117334855987494No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG6450.15093933408842003No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6420.15023729067405528No Hit
ACGCGGATCTTGACGGTTCGGCGGGTAGCCGTCGGAAGAGTCGG5900.13806853815839973No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5860.13713248027258004No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5760.1347923355580309No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG5610.1312821184862072No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5460.12777190141438347No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG5380.12589978564274415No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5290.12379365539964991No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG5200.12168752515655569No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT5200.12168752515655569No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT5080.1188793514990967No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG4980.11653920678454757No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC4930.11536913442727298No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA4630.10834870028362555No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4580.10717862792635095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)