FastQCFastQC Report
Wed 31 May 2023
EGAF00007942214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427324
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT53301.2472971328546958No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC30760.7198285141953178No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA26630.623180537484438No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG25830.6044593797680449No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG24220.5667830498638036No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG24210.5665490353923487No Hit
ATGTCCAACATGGACATCGATGGTATTAACA21000.491430390055321No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20970.4907283466409563No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG20240.4736452902247475No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17980.42075801967593673No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG17600.41186546976064997No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17300.4048450356170025No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG16370.3830816897716955No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC16030.3751251977422284No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC14680.3435332440958149No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC13830.32364201402214715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13140.307495015491758No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG13030.30492085630575394No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC12900.30187866817684006No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC12680.2967303498048319No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG11100.25975606331495543No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10820.2532036581142178No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9650.22582396495399276No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG9410.2202076176390748No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC9180.21482528479561175No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8790.2056987204088701No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8670.20289054675141108No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG8310.19446602577903419No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC8170.19118982317866537No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7870.18416938903501792No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG7710.1804251574917393No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7630.17855304172009998No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG7520.1759788825340959No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7440.1741067667624566No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7350.17200063651936237No Hit
GACCCATTCCTGGACGGCTCTCACTCTGTTA7020.16427815896135017No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6930.16217202871825592No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC6850.16029991294661664No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6820.15959786953225188No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6530.15281144986005935No Hit
GCCAACTACATCGTCCGTCAGAGCCGCGGTG6290.1471951025451414No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6240.1460250301878668No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5850.13689846580112514No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5790.13549437897239566No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5740.1343243066151211No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5570.13034606060038753No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5460.12777190141438347No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5410.1266018290571089No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5390.12613380011419906No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5270.12332562645674008No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4940.11560314889872789No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG4820.11279497524126893No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4440.10390242532598216No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4310.10086023719706827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)