FastQCFastQC Report
Wed 31 May 2023
EGAF00007942228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424880
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT48321.1372622858218793No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT21140.4975522500470721No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20830.49025607230276785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19650.4624835247599322No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC14060.3309169647900584No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13860.3262097533421201No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13390.3151478064394653No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12850.302438335530032No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12760.3003200903784598No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12350.2906703069101864No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA11850.2789022782903408No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC11600.273018263980418No Hit
GCTGACGCAAAACTGGTTTGCAACCGTTGTC9750.22947655808698927No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9690.22806439465260778No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9080.21370739973639616No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG8630.2031161739785351No Hit
CGTGTTCGTGGCCACTATCGTGCGCCATGGG7530.17722651101487477No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT7400.17416682357371494No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC7310.17204857842214272No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7210.1696949726981736No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7110.16734136697420446No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC6910.16263415552626623No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6720.15816230465072492No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT6600.15533797778196196No Hit
CGTGTTCGTGGTCACTACCGTGCTCCGTGGG6550.1541611749199774No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA6540.1539258143475805No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6470.1522782903408021No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6140.144511391451704No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5870.1381566559969874No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG5730.1348616079834306No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5620.1322726416870646No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5550.1306251176802862No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG5250.12356430050837884No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5060.1190924496328375No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5030.11838636791564677No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4800.1129730747505178No Hit
CGCAACTACACTCCGACCCCGCCACCGGTGT4780.11250235360572398No Hit
CGTGTCCGTGGCCACTACCGTGCCCCATGGG4530.10661833929580117No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG4420.10402937299943513No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG4350.10238184899265676No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4290.10096968555827526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)