FastQCFastQC Report
Sun 21 May 2023
EGAF00007942229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488802
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT21650.4429196279884288No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC18350.37540762926501936No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG16110.32958130285882625No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG15660.320375121214725No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA15120.3093277032418034No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA13810.282527485566753No Hit
CCAGGCAGAACCGTGGTGGTTACGGGATGCGCTACCAGAATGGC13700.28027708560930603No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC13490.2759808675087254No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC12700.2598189041779698No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG12550.25675017696326935No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9810.2006947598414082No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC9780.2000810143984681No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC9420.19271606908318706No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG9020.18453279651065257No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG8990.18391905106771247No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8810.18023657841007196No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA7770.15896006972148233No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT7600.15548217887815516No Hit
GAAGATGCTCGGACCGATACCAGTCCAAGACGGACGGTACTGAA6610.1352285792611323No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6530.13359192474662543No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6490.13277359748937198No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA6420.13134152478917843No Hit
TGGGTCCGGCTTGCGGGTAGCTGCGCTAGTCGGGGCGACAGCCG6280.12847737938879136No Hit
CTGCATCTGCACACCCATACGTTTCTGGTAGGCTTGCAGGTTCT6180.12643156124565774No Hit
TCATTTGAAACGCTGCATCTGGACGCCCATACGTTTCTGGTAGG6090.1245903249168375No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6050.12377199765958403No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5780.11824828867312326No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5620.11497497964410948No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG5590.11436123420116939No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG5520.11292916150097586No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5340.10924668884333534No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5340.10924668884333534No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT5320.10883752521470862No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5170.10576879800000819No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5050.10331381622824783No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG4890.10004050719923405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)