FastQCFastQC Report
Wed 31 May 2023
EGAF00007942231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451452
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA36090.7994205364025411No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA31310.6935399555212958No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT30020.6649654891328425No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC25520.5652871180103312No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23880.5289598894234604No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT23020.5099102451644915No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18840.41732011376624756No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16670.3692529881360588No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT16390.3630507783773247No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA16160.3579561060755075No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT15920.3526399262823069No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA15890.3519754038081568No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC13470.298370590893384No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13410.29704154594508386No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11720.25960677990129627No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG11120.24631633041829476No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10450.2314753284956097No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG10100.22372256629719214No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG9900.219292416469525No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9600.2126471917280242No Hit
CGCGCCGAAGTTAGATTTATTTTCGGTACCGTCCAGATCGATCA9080.2011288021760896No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG8240.18252217289988745No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA8220.18207915791712076No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7810.17299735077040307No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7510.16635212602890231No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7280.16125745372708505No Hit
AACGTGACCTTCCTCATCTTTACGCCAGTGCTGCGCCTCATCGA6720.14885303420961696No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6590.14597343682163333No Hit
ACCCAGGTCAGTACCGTTGGTGGTGCCGGTCAGCGCAGTGGTGC6590.14597343682163333No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6500.14397986939918309No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6480.14353685441641637No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG6370.1411002720111994No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6330.140214242045666No Hit
ACCCTGGTGCAGACCCAGGACTGGAGACAGTTCCGGCATGGACG6180.1368916296749156No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5880.13024640493341486No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5240.1160699254848799No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT5130.11363334307966295No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT4810.10654510335539548No Hit
GACGGTCTGGATAGCCGCGTCTACGGTGGACACCGGCGGCGGGG4790.10610208837262877No Hit
GATTGCCGGAGATGGAGTTTCCGGACGCGCCGGAGATTCCCAGG4690.10388701345879518No Hit
GGTGCTGTTGGCTTTGCCGCCGGTGGACGTCACGGTTGGTACTG4590.1016719385449616No Hit
CGGTTCATGGATCGGGCTGACGTTAGGGGTAGCAGTGTGGGAGT4580.10145043105357823No Hit
GTCAACCAGGGACACGGTTTCGCCGGTAAACAGTGGGCTGAAGC4540.1005644010880448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)