FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007942232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451452
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT80301.7787051558083695No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA61101.353410772352321No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31230.6917678955902289No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA26970.5974057042609181No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA22710.5030435129316073No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21690.48044974881050473No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20070.4445655352064007No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18380.4071307691626131No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC16960.37567670538617615No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16630.3683669581705253No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16350.3621647484117913No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15900.35219691129954017No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13530.2996996358416842No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12880.28530164890176585No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10960.242772210556161No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10810.23944959818541062No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10520.23302588093529322No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10290.227931208633476No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10150.22483010375410895No Hit
TCCCGTGAGGCACTGGAACTGCGCGACGGTG9620.21309020671079096No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8500.1882813676758548No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8020.17764900808945358No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7820.1732188582617864No Hit
AACGATGGCTCCGTAGAATGGCAGTACCAGG7580.1679026784685858No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7160.15859936383048476No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7020.1554982589511177No Hit
CCGACTGGTACGATCCCGGATATGCGTGGCC6950.15394770651143422No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6800.15062509414068384No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC6480.14353685441641637No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6040.1337905247955486No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC5570.12337967270053074No Hit
CCGAACACCTCTAGCCCGTCTATGCCGGAAC5310.11762047792456341No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT5240.1160699254848799No Hit
CGTGTTCGTGGCCACTATCGTGCGCCATGGG5070.1123042981313628No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4910.10876017826922907No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA4890.10831716328646235No Hit
GGTGAACTGGCTGCATCTGCGGCATCCCTGA4880.10809565579507899No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG4840.10720962582954556No Hit
CGTGTTCGTGGTCACTACCGTGCTCCGTGGG4840.10720962582954556No Hit
CAGGCAAGCGGTCCGGGTAACTCCTCCACCT4670.10344399847602846No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT4540.1005644010880448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)