FastQCFastQC Report
Sun 21 May 2023
EGAF00007942240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519794
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT96711.860544754268037No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG38210.7350989045660397No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG35130.6758446615389943No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG26920.5178974747688507No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22820.439020073336745No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG22350.4299780297579426No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT21980.42285982523845983No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC17240.33166985382670827No Hit
GAAGATTTCGACATCTACGTGGACTCTTTCC16370.3149324540106273No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG13770.2649126384683163No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA12360.23778650773190918No Hit
GGTCTGGTTACTGCTAAAGCTGTCATCGATG12150.2337464457073379No Hit
GAAGCAGGTGGCACCAACACCACCCCGGCGA12050.23182260664801826No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG11210.21566235854973315No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA11130.21412328730227745No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT10860.2089289218421144No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG10750.20681269887686277No Hit
ATGTCCAACATGGACATCGATGGTATTAACA10210.19642396795653663No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9890.19026768296671373No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9560.18391901407095887No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT8910.17141406018538113No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8870.17064452456165327No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC8830.1698749889379254No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA8650.16641207863115004No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT8390.16141009707691892No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG8330.16025579364132714No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT8130.15640811552268785No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC8000.1539071247455723No Hit
CCAGCGGCTGAAGCTCACGCCGCGCGTTCCG7970.1533299730277764No Hit
ACCGGCCCGACTCTGACTACCCACTCTTGCT7640.14698130413202154No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG7450.1433260099193142No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG7350.14140217085999454No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7230.13909356398881095No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC7160.1377468766472872No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA7030.13524588587017164No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6610.1271657618210291No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC6540.12581907447950536No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6440.12389523542018568No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6430.12370285151425371No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6260.1204323251134103No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6000.11543034355917922No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5990.11523795965324725No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5980.11504557574731529No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5830.11215981715833581No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5640.10850452294562847No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5520.10619591607444487No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA5490.10561876435664896No Hit
CTGGCATACCGTTCTCTGACCCGCAAAAAAC5430.1044644609210572No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5420.10427207701512523No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5410.10407969310919325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)