FastQCFastQC Report
Wed 31 May 2023
EGAF00007942278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609123
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTTTCCTGGGCGATCTGGTTGACCGTG173352.8458948356900002No Hit
TACAGGAAGGTGTCCTGACCTGGTACGAAGA64061.0516759340888457No Hit
AAGATTCTGCTGCAGCGTTACGGTGATTTCC51070.8384185131738581No Hit
GCGCTGACGGCCACCTTCTCCAACATCGTGG50990.837105149534659No Hit
ATGCACGTGATTTCTCGTAAACCGTTCAACG45680.7499306379828048No Hit
CGTACCGTAAAAGGTGGCACCATCGAGCCGC39420.647159933215459No Hit
ATGCTGTGGGCTCGTCAGGTCATTTTCATGA37380.6136691604158766No Hit
GTTGGCGGCGTAGGCGCAGGCGCAGACCCGT30550.5015407397192356No Hit
TTCTCCAGTCAGTCTGGTAGCTGGTACCTGA28030.46016978508445744No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25890.425037307735876No Hit
TCTACTCTGATCCGCCTGATGTCGGTGTTGA19040.31258054612943525No Hit
ATGGACATCTGTCTGCGTTTCTTCTGCGCGG15560.2554492278242654No Hit
CAGTCCTTTACCGAATGGCAGAACCCGGAGG14910.244778148255771No Hit
GACCAGAGCACTCTGGGCTCTAATTGGGGTG14030.2303311482245786No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12460.2045563868052922No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11990.19684037542499624No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA11500.1887960231349005No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10050.16499130717441304No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG9260.15202184123731988No Hit
CCGGCGTCTAGCGGTTTCGCAAGCGTTATGC8960.1470967275903225No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG8350.13708232984142776No Hit
GACAGCATGCGTACCATCGATCAGACCTACA8210.13478394347282896No Hit
ACTATTCTGACCTACGCACCGAAAGCAATTG8020.1316647048297306No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG7550.12394869344943468No Hit
GGTTCTTCCAAAGGTGACGTTGCAGAGGGTG7470.12263532981023537No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6840.11229259115154082No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6720.11032254569274186No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6540.10736747750454342No Hit
AACAAATCTCGCGCGGCTGAACCGACCGTCG6470.10621828432024402No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6330.10391989795164525No Hit
ATGTGCGAAGACACCATCACCTACGAATGTC6270.10293487522224576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)