FastQCFastQC Report
Wed 31 May 2023
EGAF00007942288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556873
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT92141.6545962903570473No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26110.4688681261257055No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG23130.4153550270887617No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG21430.38482742025560585No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC20810.3736938224693961No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG20760.3727959516801856No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT19820.35591598084302883No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT18840.33831771337450367No Hit
CTGTCCTCCATGGCTATCACCTTCAAACGCG17450.3133569054344527No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA17170.30832882901487413No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC17110.30725138406782154No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC15050.27025910755235033No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA12660.22734088382809006No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT12640.2269817355124059No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12550.22536556809182703No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12440.223390252355564No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12370.22213323325066936No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC12190.21890089840951166No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11820.21225665456935422No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11650.20920389388603866No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10250.1840635117881456No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT9810.17616224884309348No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9560.17167289489704116No Hit
TCTGTGCGCGCAGCTGTATTCGCCACCGCGG9550.17149332073919907No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9300.16700396679314675No Hit
GTTCTGGTTAACGAAGGTAACGGTTATGTGG9170.16466950274119954No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8850.15892312969025255No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8820.15838440721672625No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8710.15640909148046322No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA8550.15353590495498975No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8230.14778953190404273No Hit
CTGTCTTCCATGGCTATTACCTTCAAGCGTG7800.14006784311683274No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7790.13988826895899065No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC7520.13503976669725415No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7430.1334235992766753No Hit
GTCGCGGGTAAACGTGAAGGTCTGGGCGGTC7090.12731807791004413No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT7000.1257019104894653No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6970.125163188015939No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA6630.11905766664930782No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6630.11905766664930782No Hit
GAACTGGCTCGCCGTCAACAGGAAGAACGTA6590.11833937001793945No Hit
CTGTCCTCTATGGCAATCACCTTCAAACGTG6500.11672320259736062No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT6450.11582533180815016No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA6410.11510703517678177No Hit
GTCCACATCAAACTGCCGAAGCTGGCTGCAA6350.11402959022972922No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6330.11367044191404503No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6280.11277257112483456No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA6160.11061768123072944No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6140.11025853291504527No Hit
CCGGCTCTGGGCGCTCGTCGTAAGAAAAAAA5900.10594875312683504No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC5840.10487130817978245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)