FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007942305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387358
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG25410.6559823212635341No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22140.5715642893653933No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20050.5176090335038905No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA18800.48533914363457065No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18640.4812085977312977No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG18340.47346382416266086No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17700.45694164054956915No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG16820.43422363808156794No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA16740.4321583651299315No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15750.40660061235343015No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15370.39679056583315686No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11550.29817378239251546No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11110.2868147811585149No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10730.2770047346382416No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9190.23724823031923956No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8080.20859256811528354No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7510.19387749833487367No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7000.1807113832681912No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT6560.1693523820341906No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG6380.16470551789300852No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6280.16212392670346296No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5970.15412099401587162No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT5570.14379462925768927No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5410.13966408335441632No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5380.13888960599755265No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4860.1254653318119156No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT4810.12417453621714279No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT4730.12210926326550633No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG4570.11797871736223338No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4520.1166879217674606No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA4330.11178289850732398No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4290.11075026203150573No Hit
TTCCGGGTGGATGTGACCGTGATGGTCGTGATCGTGATCATGGT4210.10868498907986926No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA3990.10300548846286899No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA3950.10197285198705075No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT3890.10042389727332338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)