FastQCFastQC Report
Wed 31 May 2023
EGAF00007942316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412585
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60541.4673340039022262No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT54421.3190009331410497No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT24300.5889695456693772No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20730.5024419210586909No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16810.4074311959959766No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC14950.36234957645091315No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12110.2935152756401711No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11990.29060678405661866No Hit
CCGGATTTTGAACAGAACATTGCGACCACCA10110.24504041591429646No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9880.23946580704582088No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8950.21692499727328915No Hit
ACTCGTCTGGAAAACATCAAAACCGACCGTA8840.214258879988366No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8660.2098961426130373No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8520.20650290243222608No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8420.20407915944593236No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8140.19729267908430992No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7950.1926875674103518No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7780.18856720433365246No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7460.18081122677751252No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7370.17862985808984816No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7150.17329762352000194No Hit
GAACCGATCTACGCTACCATCGACGACCTGG6780.16432977447071515No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6750.163602651574827No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6590.15972466279675704No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6260.1517263109419877No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5970.1446974562817359No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5950.14421270768447714No Hit
TACGCAACCGACCCGGAATACGATCGTAAGC5660.1371838530242253No Hit
GCTGCTTCTAGCGATGCACTGGAAGCCCTGG5580.1352448586351903No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5560.13476011003793156No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5550.1345177357393022No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5540.13427536144067284No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5510.1335482385447847No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5410.13112449555849098No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5360.1299126240653441No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5320.1289431268708266No Hit
CAGGACCAGGCCCAGACCAAAATGGACGATA5270.12773125537767974No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5270.12773125537767974No Hit
TTCGGTCAGGCTAAAGAAAAAGCACCGTGCA5250.12724650678042101No Hit
TCCCAGGAAATCACCTTCGTTTACGAGAAAA4980.12070240071742792No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA4960.12021765212016917No Hit
TACCTGGAGACCCAGCAGAAAATCAACCACC4870.11803628343250482No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4700.11391592035580547No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC4690.11367354605717608No Hit
AAACCGGCAGCGTCCCTGCTGAAACGTAACC4600.11149217736951174No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4540.1100379315777355No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC4510.10931080868184738No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4490.10882606008458863No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4440.10761418859144177No Hit
TTCAACAAAAAACAGAGCTCCGACGTAAAAG4320.10470569700788929No Hit
TTCATCAACATGTACACCGCACACTCCCAGA4310.10446332270925991No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4230.10252432832022493No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA4210.10203957972296618No Hit
ACCAAGACTTTCGCGAAAAAACACAACCTGA4180.10131245682707805No Hit
GACAACGCGCCACGTGGCTCCCGTCGCGTAA4180.10131245682707805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)