FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007942317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437482
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT33500.7657457906839595No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC28060.6413978175102061No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG25570.5844811900832492No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG21370.4884772402064542No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA20410.4665334802346154No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18530.42356028362309767No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG18330.41898866696229786No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT17120.3913303861644593No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA16740.3826443145089398No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT15820.3616148778692609No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC15710.35910048870582106No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT14700.33601382456878226No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13750.3142986454299834No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT13640.31178425626654355No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG13310.3042410887762239No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT12500.2857260412999849No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11860.2710968679854257No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT11430.2612678921647062No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC11250.25715343716998645No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC11110.2539533055074266No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT10690.24435291051974709No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG10330.23612400053030755No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT10270.2347525155320676No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC9880.22583786304350806No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG9820.22446637804526817No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA9610.21966618055142842No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA9560.21852327638622848No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT9280.21212301306110878No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT9210.21052294722982887No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC9090.207779977233349No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8750.20000822890998946No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC8640.19749383974654958No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC8530.1949794505831097No Hit
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG8020.18332182809807032No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG7790.1780644689381506No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7580.17326427144431086No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG7540.1723499481121509No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7510.17166420561303095No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7360.1682354931174311No Hit
GTGACGACGGCGGCGTTCACCGCCGTCCGGACCTTCGCCTTCAC7350.16800691228439113No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG7270.16617826562007124No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7010.16023516396103155No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA6900.15772077479759167No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG6770.15474922396807184No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC6740.15406348146895188No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6660.15223483480463196No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG6660.15223483480463196No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6490.14834896064295217No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT6220.1421772781508725No Hit
TACCGGCAGCGGGCGGCCTGCGATATCCGCGTTGATCTTCAGCA6050.1382914039891927No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG5890.1346341106605529No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5890.1346341106605529No Hit
ACCCTCACCGGAACGAGCCGGACGAGAACCTTCACGGTGACGGC5500.12571945817199337No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5060.11566190151823388No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC4980.11383325485391398No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4900.1120046081895941No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT4830.11040454235831418No Hit
ACCCTGGTGCAGACCCAGGACTGGAGACAGTTCCGGCATGGACG4810.1099473806922342No Hit
TACCTGGATGTCCAGATCGAAGTAGAAACGGCGTGCCATGGCCG4750.10857589569399427No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT4750.10857589569399427No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA4670.10674724902967436No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG4620.10560434486447443No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG4580.10469002153231446No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG4530.10354711736711453No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT4500.10286137486799458No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG4490.1026327940349546No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4470.10217563236887461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)