FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007942318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437482
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63691.4558313256316833No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37460.8562638005677948No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG34030.777860574835079No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC27140.6203683808705273No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG24190.5529370351237308No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA23960.5476796759638111No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG23840.5449367059673312No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21900.5005920243575736No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG20830.47613387522229483No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20540.4695050310641352No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC19540.44664694776013647No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19140.4375037144385369No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC18610.4253889302874176No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15750.36001481203798097No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15590.3563575187093412No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15580.3561289378763012No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG13760.31452722626302343No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12880.29441211295550446No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12720.29075481962686467No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11980.27383983798190553No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT11630.26583950882550594No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11010.2516674971770267No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10530.2406956171911073No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA10430.23840980886070742No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA10330.23612400053030755No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10300.23543825803118756No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10200.2331524497007877No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9970.22789509054086796No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC9950.22743792887478798No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9810.22423779721222817No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9770.22332347388006824No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9610.21966618055142842No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9480.21669462972190856No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8650.19772242057958955No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8540.19520803141614967No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8390.1917793189205499No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC7790.1780644689381506No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG7590.17349285227735084No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG7500.17143562477999094No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7450.170292720614791No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7450.170292720614791No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC7410.16937839728263104No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7310.16709258895223117No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG7200.16457819978879132No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC7170.16389245728967136No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6970.15932084062887159No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6860.15680645146543173No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6730.15383490063591188No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6460.1476632181438322No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC6270.14332018231607244No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6170.14103437398567253No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6030.13783424232311273No Hit
TCCGTTTCTGAACCGAGCGAGTCCACCGGTC6030.13783424232311273No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5910.13509127232663287No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5780.132119721497113No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA5360.12251932650943352No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5340.12206216484335354No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG5280.12069067984511364No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5240.11977635651295368No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC4880.11154744652351412No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4770.10903305736007425No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA4760.10880447652703426No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA4650.10629008736359438No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4650.10629008736359438No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG4640.10606150653055439No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC4580.10469002153231446No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC4550.1040042790331945No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4500.10286137486799458No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4460.10194705153583462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)